| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new gene capable of producing a microorganism and a plant having enhanced absorption and decomposition of nitrogen oxides, by transferring the cell of the microorganism and the plant to the gene, comprising the gene for encoding a nitrous oxide reductase having a specific amino acid sequence. SOLUTION: This new gene encodes a nitrous oxide reductase comprising an amino acid sequence of the formula and is useful for improving the function of an enzyme for decomposing nitrous oxide into nitrogen or for designing the molecule of an artificial reducing catalyst and for creating a microorganism and a plant having enhanced absorption and decomposition of nitrogen oxides by transferring the gene into the cell of the microorganism and the plant. The gene is obtained by culturing Achromobacter cycloclastes, destroying the microbial cell with a surfactant, extracting a gene DNA, preparing a gene library by a conventional method, carrying out gene amplification (RCR) by using the gene of the partial sequence of the enzyme and performing a screening by the use of the prepared gene as a probe. |
