发明名称 |
USE OF MULTIVALENT CHIMERIC PEPTIDE-LOADED, MHC/IG MOLECULES TO DETECT, ACTIVATE OR SUPPRESS ANTIGEN-SPECIFIC T CELL-DEPENDENT IMMUNE RESPONSES |
摘要 |
To increase the effective affinity of soluble analogs of peptide/MHC molecules for their cognate ligands, divalent peptide/MHC complexes were constructed. Using a recombinant DNA strategy, DNA encoding the MHC class I was ligated to DNA coding for murine Ig heavy chain. MHC/Ig complexes were exploited to homogeneously load with peptides of interest. The results of flow cytometry demonstrated that the pepMHC/Ig complexes bound specifically with high affinity to cells bearing their cognate receptors. pepMHC/Ig complexes are also useful in modulating effector functions of antigen-specific T cells. These pepMHC/Ig complexes are useful for studying TCR/MHC interactions and lymphocyte tracking and have uses as specific regulators of immune responses.
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申请公布号 |
CA2303396(A1) |
申请公布日期 |
1999.03.18 |
申请号 |
CA19982303396 |
申请日期 |
1998.09.11 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
PARDOLL, DREW;SCHNECK, JONATHAN;SLANSKY, JILL;O'HERRIN, SEAN M.;GRETEN, TIM |
分类号 |
G01N33/53;A61K35/12;A61K38/00;A61K39/00;A61K39/145;A61K39/21;A61K39/385;A61K39/395;A61K45/00;A61K47/48;A61P31/00;A61P35/00;A61P37/02;A61P43/00;C07K14/11;C07K14/15;C07K14/705;C07K14/725;C07K14/74;C07K19/00;C12N15/09;C12P21/08;(IPC1-7):C12N15/85;A61K38/17 |
主分类号 |
G01N33/53 |
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