发明名称 |
METHOD OF INACTIVATING GENES WHICH ENCODE ENZYMES OF PHENYL ACETATE CATABOLISM, TRANSFORMED STRAINS AND INACTIVATION PLASMIDS |
摘要 |
Procedure for the inactivation of genes that code for phenylacetate catabolism enzymes, plasmids containing them, and strains transformed with them. The procedure is preferably applied to the phacA gene of A. nidulans and to the pahA gene of P. chrysogenum, genes which code respectively for enzymes competing for this substrate with penicillin biosynthesis enzymes. The non-expression of these enzymes has a beneficial effect on the synthesis of this antibiotic, increasing its yield, with a lower phenylacetate demand per unit of culture. <IMAGE> |
申请公布号 |
CZ9804172(A3) |
申请公布日期 |
1999.03.17 |
申请号 |
CZ19980004172 |
申请日期 |
1998.04.17 |
申请人 |
ANTIBIOTICOS |
发明人 |
FERNANDEZ CANON JOSE MANUEL;RODRIGUEZ SAIZ MARTA;DIEZ GARCIA BRUNO;BARREDO FUENTE JOSE LUIS;VITALLER ALBA ALEJANDRO;SALTO MALDONADO FRANCISCO;PENALVA SOTO MIGUEL ANGEL;MINGOT ASCENCAO JOSE MANUEL |
分类号 |
C12N15/09;C12N1/15;C12N9/02;C12N9/06;C12N15/53;C12N15/90;C12P37/00;C12R1/66;C12R1/82 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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