Polyelectrolyte treated glass for enzyme immobilization and protein purification

摘要

Materials and methods for selectively adsorbing proteins are disclosed. Under the invention, controlled pore glass is treated with a polyelectrolyte layer which comprises at least one polymer having a cationic portion which is electrostatically attracted to the porous outer surface of the glass. The constituents of the polyelectrolyte are preferably selected to have an affinity for the predetermined protein to facilitate adsorption thereof on the polyelectrolyte treated glass. Advantageously, the materials of the present invention can be utilized to immobilize enzymes and also to separate proteins from other components in a medium.