发明名称 |
Geranylgeraniol-18-hydroxylase from croton sublyratus |
摘要 |
Geranylgeraniol-18-hydroxylase, which catalyzes the C-18 hydroxylation of geranylgeraniol to form plaunotol, is extracted from Croton sublyratus using an extraction buffer and is purified using a differential centrifugation separation technique. The purified enzyme is used to convert geranylgeraniol to plaunotol, an anti-peptic ulcer agent. The enzymatic activity of the hydroxylase can be increased by a factor of about three by heating the enzyme in boiling water for about 30 minutes. The enzyme activity is also increased by adding a coenzyme, NADPH, which increases enzyme activity by a factor of about 2 or NADH, which increases activity by a factor of about 1.4. The pH optimum for hydroxylase enzyme activity is 5.0 The hydroxylase is a complex enzyme with a diameter of about 20 to 40 nm.
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申请公布号 |
US5879916(A) |
申请公布日期 |
1999.03.09 |
申请号 |
US19970923789 |
申请日期 |
1997.09.04 |
申请人 |
THE THAILAND RESEARCH FUND |
发明人 |
DE-EKNAMKUL, WANCHAI;TANSAKUL, PIMPIMON |
分类号 |
C12N9/02;(IPC1-7):C12P7/02;C12P7/04 |
主分类号 |
C12N9/02 |
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