| 摘要 |
Methods for detecting clear cell type renal carcinoma in a mammal are disclosed. One method includes providing a biological sample from the mammal and determining concentration of Na, K-ATPase's beta -subunit in the biological sample. The concentration of Na, K-ATPase's beta -subunit in the biological sample is then correlated with the presence of clear cell type renal carcinoma in the mammal. In another method, a biological sample from the mammal is provided and contacted with an agent capable of cleaving N-linked sugar residues, lactosamine residues, sialic acid residues, or combinations thereof from Na, K-ATPase's beta -subunit under conditions effective to cleave N-linked sugar residues, lactosamine residues, sialic acid residues, or combinations thereof from Na, K-ATPase's beta -subunit in normal kidney tissue. The concentration of N-linked sugar residues, lactosamine residues, sialic acid residues, or combinations thereof in Na, K-ATPase's beta -subunit in the biological sample is determined and correlated with the presence of clear cell type renal carcinoma in the mammal. In still another method, a tissue section from the mammal and a biological agent which binds to Na, K-ATPase are provided. The tissue section is contacted with the biological agent under conditions effective to permit binding of the biological agent to Na, K-ATPase in the tissue section. The method further includes detecting the Na, K-ATPase's spatial distribution in the tissue section by detecting the biological agent's spatial distribution in the tissue and correlating the Na, K-ATPase's spatial distribution in the tissue section to the presence of clear cell type renal carcinoma in the mammal. In addition, a kit for detecting clear cell type renal carcinoma is disclosed. The kit includes an agent capable of cleaving N-linked sugar residues, lactosamine residues, sialic acid, or combinations thereof and a biological agent which binds to Na, K-ATPase. |
