发明名称 |
En metode for Õ fremstille birnavirus fra syntetiske RNA-transkripter |
摘要 |
<p>A system for the generation of live Birnavirus such as infectious bursal disease virus (IBDV), a segmented double-stranded (ds)RNA virus of the Birnavirdae family, using synthetic transcripts derived from cloned DNA has been developed. Independent full-length cDMA clones were constructed which contained the entire coding and noncoding regions of RNA segments A and B of IBDV, respectively. Synthetic RNAs of both segments were produced by in viro transcription of linearized plasmids with T7 RNA polymerase. Transfection of Vero cells with combined plus-sense transcripts of both segments generated infectious virus as early as 36 hours post-transfection. The development of a reverse genetics system for dsRNA viruses will greatly facilitate studies of the regulation of viral gene expression pathogenesis, and design of a new generation of live and inactivated vaccines.</p> |
申请公布号 |
NO991074(D0) |
申请公布日期 |
1999.03.04 |
申请号 |
NO19990001074 |
申请日期 |
1999.03.04 |
申请人 |
UNIVERSITY OF MARYLAND - BIOTECHNOLOGY INSTITUTE |
发明人 |
VAKHARIA, VIKRAM N.;MUNDT, EGBERT |
分类号 |
C12N15/09;A61K39/00;A61K39/12;A61P31/12;C07K14/08;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N7/00;C12P21/02;(IPC1-7):C12N |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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