A PROCESS TO STUDY CHANGES IN GENE EXPRESSION IN GRANULOCYTIC CELLS
摘要
The present invention comprises a method to identify granulocytic cell genes that are differentially expressed upon exposure to a pathogen or in a sterile inflammatory disease by preparing a gene expression profile of a granulocytic cell population exposed to a pathogen or isolated from a subject having a sterile inflammatory disease and comparing that profile to a profile prepared from quiescent granulocytic cells. The present invention is particularly useful for identifying cytokine genes, genes encoding cell surface receptors and genes encoding intermediary signaling molecules. The invention also includes methods to identify a therapeutic agent that modulates the expression of at least one gene in a granulocyte population. Genes which are differentially expressed during neutrophil contact with a pathogen, such as a virulent bacteria, or that are differentially expressed in a subject having a sterile inflammatory disease are of particular importance.
申请公布号
WO9910536(A1)
申请公布日期
1999.03.04
申请号
WO1998US17284
申请日期
1998.08.21
申请人
YALE UNIVERSITY;YERRAMILLI, SUBRAHMANYAM, V.;PRASHAR, YATINDRA;NEWBURGER, PETER;GOGUEN, JON;WEISSMAN, SHERMAN, M.
发明人
YERRAMILLI, SUBRAHMANYAM, V.;PRASHAR, YATINDRA;NEWBURGER, PETER;GOGUEN, JON;WEISSMAN, SHERMAN, M.