发明名称 |
Synthetic eukaryotic promoters containing two inducible elements |
摘要 |
Synthetic inducible eukaryotic promoters for the regulation of transcription of a gene achieve improved levels of protein expression and lower basal levels of gene expression. Such promoters contain at least two different classes of inducible elements, usually by modification of a native promoter containing one of the inducible elements by inserting the other of the inducible elements. In embodiments, additional metal responsive elements IR:Es) and/or glucocorticoid responsive elements (GREs) are provided to native promoters, particularly the hMT-IIA and MMTV-LTR promoters. One or more constitutive elements may be functionally disabled to provide the lower basal levels of gene expression.
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申请公布号 |
US5877018(A) |
申请公布日期 |
1999.03.02 |
申请号 |
US19960633289 |
申请日期 |
1996.04.16 |
申请人 |
CONNAUGHT LABORATORIES LIMITED |
发明人 |
FILMUS, JORGE;KLEIN, MICHEL |
分类号 |
C12N15/85;(IPC1-7):C12N15/85;C12N15/63;C07H71/04;C12Q1/68 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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