| 摘要 |
A method of determining the concentration of an analyte with the use of a bioelement comprises the steps of introducing into a chamber comprising a first and a second surface disposed opposite one another, a sample containing the analyte and a quantity of a bioelement selected for its ability to interact with the analyte bringing about a change in the chemical, physical and/or chemico-physical characteristics of the analyte-bioelement system, and providing transducer means on at least one of the surfaces of the chamber for converting the change into a physical magnitude variable over time in dependence on the analyte-bioelement interaction and correlating the physical magnitude with the value of the concentration of the analyte. The analyte-bioelement interaction takes place within the depth of the chamber which is equal to the distance between the surfaces and which is selected so as to be less than or equal to 500 micrometres in order to ensure rapid diffusion of the analyte between the surfaces, the quantity of the selected bioelement is such as to ensure that all of the analyte interacts with the bioelement, and the concentration of the analyte is determined after all of the analyte has interacted with the bioelement when the physical magnitude tends to adopt an approximately constant value which is proportional to the concentration of the analyte and is also independent of the activity of the bioelement. |
