摘要 |
In apparatus, such as a fluorometric microscope, for measuring two different fluorescences in a sample, the sample is irradiated by light modulated at a first frequency and having a first wavelength for causing a first fluorescence and also is irradiated by light modulated at a second frequency different from the first frequency and having a second wavelength for causing a second fluorescence. A photoelectric element is responsive to the light emitted by the sample during both fluorescences for generating an electrical signal representative of the combined intensities of the two fluorescences. From this electrical signal, a demodulator derives first and second signal components having the first and second frequencies, respectively, and having amplitudes representative of the individual intensities of the first and second fluorescences, respectively.
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