A method for determining the presence and/or amount of microorganisms and/or their intracellular material present in a sample comrpising estimating the amount of adenylate kinase therein by its ability to convert adenosine diphosphate (ADP) to adenosine triphosphate (ATP) and relating that to the presence/or amount of organism and/or intracellular material. The method provides improved sensitivity over existing luciferase/luciferin assays. Reagents including purified ADP and adenyl kinase free luciferase are provided together with test kits including these and apparatus for automated operation of the method.
申请公布号
DE69412790(T2)
申请公布日期
1999.02.11
申请号
DE1994612790T
申请日期
1994.01.21
申请人
THE SECRETARY OF STATE FOR DEFENCE IN HER MAJESTY'S GOVERNMENT OF THE UNITED KINGDOM OF GREAT BRITAIN AND NORTHERN IRELAND, LONDON, GB
发明人
SQUIRRELL, DAVID JAMES, SALISBURY, WILTSHIRE SP4 0JQ, GB