发明名称 |
Amplification karyotyping |
摘要 |
A method is disclosed for determining the chromosomal identity of a sample of genomic DNA. The genomic DNA is amplified and labeled by polymerase chain reaction using primers substantially complementary to interspersed repetitive DNA sequences. The amplified and labeled genomic DNA fragments are then contacted with chromosomal DNA of known identity under conditions in which the chromosome-specific, but not the interspersed repetitive DNA sequences, of the amplified and labeled genomic DNA fragments are available for hybridization. Specific hybridization to chromosomal DNA of known identity determines the chromosomal identity of the sample of genomic DNA.
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申请公布号 |
US5869237(A) |
申请公布日期 |
1999.02.09 |
申请号 |
US19940343358 |
申请日期 |
1994.11.22 |
申请人 |
YALE UNIVERSITY |
发明人 |
WARD, DAVID C.;LICHTER, PETER |
分类号 |
C12P19/34;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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