发明名称 |
Method for the identification of peptides that recognize specific DNA sequences |
摘要 |
A method for identifying biologically significant peptide-DNA binding interactions and sequence-specific DNA-binding peptides in vivo using combinatorial oligonucleotide libraries is disclosed. Target DNA sequences include promoter sequences, 5' and 3' regulatory sequences, and exon and intron regulatory sequences. Preferably, a yeast combinatorial library is employed. Peptides that bind to specific DNA sequences, identified by the above method, are disclosed. Also disclosed is a method for identifying nontoxic compounds that inhibit gene expression in host cells in vivo.
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申请公布号 |
US5869250(A) |
申请公布日期 |
1999.02.09 |
申请号 |
US19960753829 |
申请日期 |
1996.12.02 |
申请人 |
THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
发明人 |
CHENG, XIAOJUN;JULIANO, R. L. |
分类号 |
C07K14/00;C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C07K14/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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