| 摘要 |
PCT No. PCT/JP97/01477 Sec. 371 Date Jul. 15, 1997 Sec. 102(e) Date Jul. 15, 1997 PCT Filed Apr. 28, 1997The present invention herein provides a method for collecting insect hemolymph which permits the collection of hemolymph, at a time, from a vast number of insect bodies, which does not cause any scattering of the hemolymph during collection thereof, which may widely be used, which permits the collection of the hemolymph free of unnecessary tissues or the like and which can inhibit the melanization of the collected hemolymph. The insect hemolymph can be collected by freezing anesthetized lepidopterous insects, piercing the epidermis of the frozen insects without damaging the alimentary canals thereof and then thawing them in a buffer solution containing a melanization-inhibitory agent to thus discharge the hemolymph of the insects into the buffer solution through the holes while making use of the self-contraction phenomenon caused during the thawing process. |
