GHRH EXPRESSION SYSTEM AND METHODS OF USE

摘要

This invention relates to gene delivery and expression, including gene therapy, by using vectors which establish controlled expression of recombinant GHRH genes within tissues at certain levels. The expression vectors of the present invention can also be regulated by a regulatory system and/or constructed with a coating. The invention concerns the use of plasmid cDNA containing nucleotide sequences encoding species-specific GHRH polypeptides and teaches expression of these polypeptides in a variety of animal species and animal tissues to provide stimulation of GH at physiological levels, sufficient to enhance growth and improve the immune systems of agricultural and other domesticated species, including household pets. The invention also concerns a novel method for reducing the rate of proteolytic degradation of secreted GHRH.