发明名称 |
DETECTION OF PURINE AND PYRIMIDINE NUCLEOTIDES AND UNDERIVATIZED NUCLEIC ACIDS BY SINUSOIDAL VOLTAMMETRY |
摘要 |
Sinusoidal voltammetry was employed to detect both purine and pyrimidine-based nucleic acids. Adenine and cytosine, representing these two classes of nucleic acids, could be detected with nanomolar detection limits at a copper electrode under these conditions, where the sensitivity for adenine was much higher than that for cytosine. Detection limits for purine-containing nucleotides (e.g., adenosine 5'-monophosphate (AMP), adenosine 5'-diphosphate (ADP), and adenosine 5'-triphosphate (ATP)) were on the order of 70-200 nM using this method. These detection limits are achieved for native nucleotides and are over two orders of magnitude lower than those found with UV absorbance detection. Pyrimidine-based nucleotides could also be detected with high sensitivity due to the presence of a sugar backbone which is electroactive at the copper surface. |
申请公布号 |
WO9904250(A1) |
申请公布日期 |
1999.01.28 |
申请号 |
WO1998US14164 |
申请日期 |
1998.07.10 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
KUHR, WERNER, G.;SINGHAL, PANKAJ |
分类号 |
G01N27/416;G01N27/49;(IPC1-7):G01N27/26 |
主分类号 |
G01N27/416 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|