The invention relates to the characterization and use of powerful viral promoters for expressing genes in bacteria and fungi, especially yeasts. The invention is based on a surprising discovery, namely that CFDV-DNA (DNA of the coconut foliar decay virus) and fragments of CFDV-DNA contain a region active as promoter even in bacteria and fungi, especially yeasts, although they originate from a virus which infects monocotyledonous plants. The activity of these promoters in E. coli is markedly greater than that of the CaMV 35S promoter which is also active in bacteria.
申请公布号
WO9904020(A1)
申请公布日期
1999.01.28
申请号
WO1998EP04345
申请日期
1998.07.13
申请人
MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V., BERLIN;ROHDE, WOLFGANG;BECKER, DIETER;RANDLES, JOHN, W.;HEHN, ALAIN;SALAMINI, FRANCESCO
发明人
ROHDE, WOLFGANG;BECKER, DIETER;RANDLES, JOHN, W.;HEHN, ALAIN;SALAMINI, FRANCESCO