| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for producing organisms of controlled spermatogenesis useful for test animals or the like, capable of controlling spermatogenesis, producing sterilized male animals and providing an index for spermatogenesis-related diseases by damaging Mea-2 gene totally or partially. SOLUTION: This method damages, totally or partially, Mea-2 gene containing a base sequence shown by the formula or the one hybridizable with the above, and also an intron. It is preferable that the damaged section in the Mea-2 gene is in the boundary between the intron and exon, and has a size of 1 bp to 1 Kbp. It is also preferable that the male organism (except human), controlled in spermatogenesis by totally or partially damaging its Mea-2 gene, is transplanted with a spermatogonia of the similar or dissimilar species having a target gene, e.g. a gene capable of coding a physiologically active material, to make it possible to form the spermatogonia-derived sperm in the individium having the damaged Mea-2 gene and to breed the organism having a target gene by mating the above male individium with a female individium. |
