| 摘要 |
The invention relates to cells for use in gene therapy, obtainable by a) isolation of cells from the blood or cell-containing fluids of the body; b) culturing of the cells obtained in step a) in a cell culture medium comprising gangliosides, phospholipids, glycolipids and/or growth factors; c) alternatively immortalization of the cells obtained in step a) or b) by transformation with an oncogene, activation of an oncogene or inactivation of a suppressor gene; and d) alternatively transfection of the cells obtained in step a) and b) or in step c) with a nucleic acid construct for gene therapy, comprising an effector gene which can be activated target cell-specifically, cell cycle-specifically, virus-specifically and/or by hypoxia by suitable promoter systems; and to the use of these cells for the production of a therapeutic for the treatment of a disorder selected from the group comprising tumors, leukemia, autoimmune disorders, allergies, arthritides, inflammations, organ rejections, transplant-versus-host reactions, blood clotting disorders, circulation disorders, anemia, infections, hormone disorders and CNS damage.
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