OSTEOCLAST-BASED CELL

摘要

PROBLEM TO BE SOLVED: To obtain a new osteoclast precursor cell useful for detection and screening of a differentiation promotion factor to an osteoclast-based cell, by culturing a hemopoietic precursor cell under specific conditions. SOLUTION: A hemopoietic precursor cell is cultured in the presence of (A) M-CSF(macrophage colony stimulating factor) and (B) a stroma cell derived from a skull to prepare an osteoclast precursor cell(Mouse Calvarial cells Initiating Osteoclast Precursors) negative for calcitonin receptor and tartaric acid- resistant acid phosphatase. The cell is differentiated into a cell having the character of an osteoclast (tartaric acid-resistant acid phosphatase positive cell) in a culture system in the presence of a stroma cell in an extremely short time.