PEROXIDASE-CATALYSED FLUORESCENCE

摘要

An assay method comprises bringing together a leuco-dye and an oxidant and a peroxidase which catalyses oxidation of the leuco-dye to a fluorescent dye. The assay is performed in the presence of an enhancer which is a substituted aromatic hydroxy or amine or borate compound which enhances fluorescent light output. The dye may be for example a fluorescein or rhodamine or oxazine dye. Preferred enhancers include 6-hydroxybenzothiazole and 6-hydroxy-2-naphthoic acid.