发明名称 |
METHODS FOR RAPIDLY DETECTING METHICILLIN RESISTANT STAPHYLOCOCCI |
摘要 |
Compositions and methods are provided for determining the presence of an antibiotic resistant mecA gene in a biological sample, comprising the general steps of (a) treating cells contained within a biological sample to expose target single-stranded nucleic acid molecules; (b) reacting the target singlestranded nucleic acids with a scissile link-containing nucleic acid which is complementary to a portion of an antibiotic resistant mecA gene, and with an enzyme which cleaves double-stranded target-probe complexes, under conditions which allow the target and probe to hybridize to each other to form a doublestranded target-probe complex, the enzyme molecule being capable of cleaving the scissile link of the target-probe complex such that one or more fragments of the nucleic acid probe released from said complex; and (c) determining whether cleaved portions of the detecting probe fragments released from said nucleic acid probe are produced, and thereby detecting the presence of the antibiotic resistant mecA gene.
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申请公布号 |
CA2295331(A1) |
申请公布日期 |
1999.01.14 |
申请号 |
CA19982295331 |
申请日期 |
1998.07.03 |
申请人 |
ID BIOMEDICAL CORPORATION |
发明人 |
CLONEY, LYNN P.;BEKKAOUI, FAOUZI |
分类号 |
C12N15/09;C12Q1/68;C12R1/445;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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