BMP2-induced cDNA and its use

摘要

The recombinant expression of human BMP2, BMP4 - BMP7 in murine mesenchymal C3H10T1/2 progenitors mediates differentiation into three mesenchymal Iineages in different efficiencies: the osteogenic, the chondrogenic and the adipogenic lineage. This developmental in vitro model was used to identify and to characterize cDNAs involved during the manifestation of these lineages in vivo. By subtractive cloning an as yet undescribed cDNA, 29A has been cloned which encodes a putative secreted factor which is expressed in developing osteo- /chondrogenic tissues of vertebrae, ribs, tooth and the limb bud.