IMPROVED BACILLUS THURINGIENSIS TOXIN

摘要

New improved Cry9C proteins, having significantly increased toxicity, and DNA sequences encoding these proteins, were designed. Analysis of amino acid positions in domain II of the Cry9C protein by protein mutagenesis identified amino acids involved in insect toxicity. Random replacement of these amino acids identifies proteins with improved toxicity. A combination of identified improved amino acids in a single protein yields modified Cry9C proteins with significantly improved toxicity.