A membrane filter pipette tip (34) comprising a hollow tube (36) having a relatively large open upper end for seating on a lower end of a pipette tip mounting shaft of a pipette device and having a relatively small lower open end for passing fluid into the lower end of the tube upon operation of the pipette device in an aspiration mode. A porous membrane (32) is secured within the tube across an open channel (42) within the tube extending between the open upper and lower ends thereof and having a fluid initiation/penetration pressure which is greater than the maximum vacuum pressure which the pipette device to which the tube is secured is capable of generating.
