摘要 |
<p>3'-Monophosphorylating oligonucleotides are provided, which stability in serum and endocellular is markedly higher than that of non-modified oligonucleotides and a little higher than that of phosphorothioate-modified oligonucleotides demonstrated by studying them in vitro and cell, which ability to uptake by cell is higher than that of non-modified oligonucleotides and phosphorothioate-modified oligonucleotides. Its products of metabolic degradation do not have any toxycity and side effect, due to 3'-monophosphorylating oligonucleotides not containing non-natural modification elements. Therefore, when used as therapeutic agents, 3'-monophosphorylating oligonucleotides are more advantageous and safer than phosphorothioate-modified oligonucleotides.</p> |