DNA, VECTOR AND MICROORGANISM CONTAINING GENE OF PEPTIDE-TRANSPORTING PROTEIN

摘要

PROBLEM TO BE SOLVED: To obtain a DNA that has high heat stability and is useful for production of peptide-transporting protein that can specifically take in di- and tri- peptide by incorporating the gene of the peptide-transporting protein originating from Lactobacillus helveticus into the vector. SOLUTION: The gene of a peptide-transporting protein originating from Lactobacillus helveticus as Lactobacillus helveticus SBT-2171 (FERM P-14381) or a peptide-transporting protein gene that is obtained by cleaving the chromosomal gene of Lactobacillus helveticus with a restriction enzyme Hpa I is included. This DNA is incorporated into a vector, a microorganism is transformed with this vector and cultured. Then, the resultant peptide-transporting protein is added to prepare membrane vesicles.