摘要 |
PROBLEM TO BE SOLVED: To inexpensively obtain an antibody Fab fragment that is useful for treatment and diagnosis of allergic symptoms in a large amount by introducing a vector including an antibody L-chain gene and H-chain VH and CH1 region gene into Pichia pastoris and expressing them. SOLUTION: Two kinds of expression cassettes of the antibody L-chain gene and the H-chain VH and CH1 region gene are prepared, respectively, and both of them are linked to a vector. In addition, the gene encoding the area participating the secretion in these genes (the signal peptide) is replaced with the signal sequence of the phosphatase (PHO1) gene originating from a yeast, Pichia pastoris, to prepare a recombinant expression vector. Then, the recombinant expression vector is introduced into the host cells of a yeast. Pichia pastoris to express the secretion of the antibody Fab fragment as an associated expression products whereby the objective human type Ig E-high affinity receptor antibody Fab fragment are inexpensively obtained in a large amount that is useful for treatment and diagnosis of allergic symptoms. |