发明名称 |
D-amino acid oxidase promoter from t. variabilis |
摘要 |
A D-amino acid oxidase promoter isolated from T. variabilis has been incorporated into S. cerevisiae expression vectors to increase the expression of heterologous genes. The heterologous promoter has been placed upstream of selective markers, auxotrophic nutritional and/or dominant selectable markers. Because the promoter is from another yeast specie, it is weakly recognized by and S. cerevisiae. To compensate for the weak transcripts of selectable marker genes transcribed from the weak heterologous promoter, the plasmid containing the gene of the interest is amplified leading to a higher expression of protein of interest. |
申请公布号 |
AU7823598(A) |
申请公布日期 |
1999.01.04 |
申请号 |
AU19980078235 |
申请日期 |
1998.06.08 |
申请人 |
BRISTOL-MYERS SQUIBB COMPANY |
发明人 |
SUO W. LIU;THOMAS FRANCESCHINI |
分类号 |
C12N15/09;C07K14/495;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/06;C12N9/16;C12N9/18;C12N9/86;C12N15/81 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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