发明名称 |
Process for preparing recombinant proteins using highly efficient expression vector from (saccharomyces cerevisiae) |
摘要 |
This invention concerns a method of producing human granulocyte colony-stimulating factor (hGCSF) and human growth hormone (hGH) from least by using recombinant DNA technology. More specifically, this invention relates to a method of producing hGCSF or hGH by using yeast expression vector which contains: hybrid promoters comprising promoters of two different yeast-derived genes, yeast killer toxin leader peptide, and amino terminus of IL-1beta. In addition, this invention relates to a method of producing hGCSF by using expression vector which contains promoter and secretion signal of HSP 150. |
申请公布号 |
AU3049297(A) |
申请公布日期 |
1998.12.30 |
申请号 |
AU19970030492 |
申请日期 |
1997.05.27 |
申请人 |
HANIL SYNTHETIC FIBER CO., LTD. |
发明人 |
KI-RYONG JANG;JAE-WOONG MOON;CHEON-SOON BAE;DOO-SUK YANG;JEE-WON LEE;BAIK-LIN SEONG |
分类号 |
C12N15/09;C07K14/39;C07K14/53;C07K14/545;C07K14/61;C12N1/19;C12N15/12;C12N15/18;C12N15/19;C12N15/81;C12P21/02;C12R1/865 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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