发明名称 |
Glycosylation of peptides using glycosyl phosphite reagents |
摘要 |
A method for synthesizing glycosylated peptides employs a blocked carbohydrate donor and a blocked peptide acceptor. The carbohydrate donor includes an acid labile phosphite leaving group attached to the anomeric carbon. The blocked peptide acceptor includes an unprotected hydroxyl group susceptible to electrophilic attack. Serine hydroxyl is preferred. The reaction is initiated by the addition of a Lewis acid so as to activate the acid labile phosphite leaving group on the carbohydrate donor. The substitution reaction may be conducted at -78 DEG C. in a solvent such as Et3N which favors the formation of glycosylated peptide products.
|
申请公布号 |
US5854391(A) |
申请公布日期 |
1998.12.29 |
申请号 |
US19930033513 |
申请日期 |
1993.03.18 |
申请人 |
THE SCRIPPS RESEARCH INSTITUTE |
发明人 |
WONG, CHI-HUEY;KONDO, HIROSATO |
分类号 |
C07K1/107;C07K9/00;(IPC1-7):C07K1/107 |
主分类号 |
C07K1/107 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|