发明名称 |
Real time homogeneous nucleotide assay |
摘要 |
A method for performing real time, homogeneous assay of a target nucleic acid comprising annealing a labeled, ribo-oligonucleotide probe to the target DNA sequence and degrading a portion of the probe with RNase H to release labeled fragments. Sequential measurements of the released fragments permits kinetic characterization of the presence of the target sequence. Preferably, the assay is integrated into a polymerase chain reaction so that target amplification can be detected in real time.
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申请公布号 |
US5853990(A) |
申请公布日期 |
1998.12.29 |
申请号 |
US19960687662 |
申请日期 |
1996.07.26 |
申请人 |
WINGER, EDWARD E. |
发明人 |
WINGER, EDWARD E.;KESSLER, DONALD J.;HARGROVE, DAVID E. |
分类号 |
C07H21/00;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;C07H21/04 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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