发明名称 PROCESS FOR LYSING MYCOBACTERIA
摘要 The invention provides a rapid process for lysing Mycobacteria. In one embodiment is provided a process for lysing Mycobacteria which comprises exposing the bacteria to a lysis effective amount of heat. The process of the invention is particularly advantageous since only one step is involved, it is expedient compared to prior methods, and little instrumentation is necessary. By practicing the present invention it is possible to lyse Mycobacteria with minimal effort. In addition, practicing the invention results in liberating cellular components including deoxyribonucleic acid (DNA) from Mycobacteria. Not only is DNA liberated, but the DNA is suited for subsequent analysis by way of probe hybridization, restriction enzyme analysis, and the like.
申请公布号 CA2084533(C) 申请公布日期 1998.12.29
申请号 CA19922084533 申请日期 1992.12.04
申请人 发明人 ROBSON, JILLIAN A.;HOWARD, ADRIANN J.;KEATING, WILLIAM E.;DOWN, JAMES A.
分类号 C12N1/06;C12N15/09;C12Q1/68;C12R1/32;(IPC1-7):C12N1/06;C12N15/10 主分类号 C12N1/06
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