| 摘要 |
The invention provides selectable fusion genes comprising a dominant positive se lectable gene fused to and in reading frame with a negative selectable gene. The selectable fusion gene encodes a sing le bifunctional fusion protein which is capable of conferring a dominant positive selectable phenotype and a negative selectable ph enotype on a cellular host. A dominant positive selectable phenotype is conferred, for example, by the hph gene for hygromycin B resistance (Hm~). A dominant negative selectable phenotype is conferred, for example, by the HSV-I TK gene for gansiclovir sensit ivity (GCV5). The present invention also provides recombinant expression vectors, for example, retroviral vectors, w hich include selectable fusion genes, and cells transduced with the recombinant expression vectors. The bifunctional selec table fusion genes are expressed and regulated as a single genetic entity, permitting co-regulation and co-expression with a high degree of efficiency.
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