Verfahren zur Immobilisierung eines Nukleinsäurefragments durch passive Fixierung auf einen Festträger, daraus erhaltener Festträger und seine Verwendung

摘要

Method for immoblising, by passive adsorption, on a solid support, a nucleic acid fragment containing less than 100 nucleotides, in which the said fragment is deposited on the said support in the form of a derivative resulting from the covalent coupling of the said fragment with a ligand having a molecular mass of less than 5000 and containing at least one polar functional group, the said derivative not being capable of reacting with the said support with the formation of a covalent bond under the conditions of the said deposition, it being understood that when the said ligand is a nucleotide or oligonucleotide, it contains at least one nucleotide which is modified so as to introduce the said polar functional group, and solid support thus obtained. This support can be used especially as a capture probe for the detection of a nucleotide target.