Inhibition of hiv replication using a mutated transfer rna primer

摘要

Modified tRNA molecules are provided which inhibit HIV-1 replication. A tRNA molecule has a modified aminoacyl acceptor stem with a 3' segment which has reduced complementarity to the HIV primer binding site can initiate aberrant reverse transcription and/or interfere with reverse transcription. tRNA molecules which are modified in regions outside the acceptor stem which interact with reverse trancriptase or the HIV-1 RNA template can also inhibit HIV-1 replication. Methods are disclosed for introducing the modified tRNA molecules into human cells.