A restriction site indexing method for selectively amplifying any fragment generated by a Class II restriction enzyme includes adaptors specific to fragment ends containing adaptor indexing sequences complementary to fragment indexing sequences near the termini of fragments generated by Class II enzyme cleavage. A method for combinatorial indexing facilitates amplification of restriction fragments whose sequence is not known.
申请公布号
WO9840518(A3)
申请公布日期
1998.12.17
申请号
WO1998US04819
申请日期
1998.03.11
申请人
WISCONSIN ALUMNI RESEARCH FOUNDATION;GUILFOYLE, RICHARD, A.;GUO, ZHEN