发明名称 |
Cell ablation using trans-splicing ribozymes |
摘要 |
The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described. |
申请公布号 |
US5849548(A) |
申请公布日期 |
1998.12.15 |
申请号 |
US19950488031 |
申请日期 |
1995.06.07 |
申请人 |
THE GENERAL HOSPITAL CORPORATION;THE PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
HASELOFF, JAMES;BRAND, ANDREA;PERRIMON, NORBERT;GOODMAN, HOWARD M. |
分类号 |
C12N1/21;A61K38/00;C07K7/06;C12N;C12N5/10;C12N7/00;C12N9/10;C12N15/09;C12N15/113;C12N15/52;C12N15/66;C12N15/82;C12N15/85;C12N15/87;C12N15/90;(IPC1-7):C12N15/63;C12N15/70;C12N15/79;C12N15/10 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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