发明名称 |
TESTING FOR INFESTATION OF RAPESEED AND OTHER CRUCIFERAE BY THE FUNGUS LEPTOSPHAERIA MACULANS (BLACKLEG INFESTATION) |
摘要 |
<p>A method of testing for infestation of tissue of rape or other Cruciferae with a virulent strain of Leptosphaeria maculans. The method involves isolating DNA of L. maculans from the tissue; subjecting the isolated DNA to amplification by the polymerase chain reaction (PCR) using sets of primers derived from LMR1 ¢SEQ ID NO:11! (Genbank accession number M77515), a repetitive element of L. maculans specific to virulent strains of the fungus, to form a product containing amplified L. maculans DNA; preferably separating the amplified L. maculans DNA from the product; and detecting the presence of the amplified separated L. maculans DNA. The invention also relates to a method of deriving DNA of L. maculans from plant tissue for amplification by PCR, to the oligonucleotides forming the primers used in the PCR and to a diagnostic test kit which makes use of the method of testing.</p> |
申请公布号 |
CA2092115(C) |
申请公布日期 |
1998.12.15 |
申请号 |
CA19932092115 |
申请日期 |
1993.03.22 |
申请人 |
HER MAJESTY IN RIGHT OF CANADA AS REPRESENTED BY THE NATIONAL RESEARCH COUNCIL OF CANADA;NATIONAL RESEARCH COUNCIL OF CANADA |
发明人 |
TAYLOR, JANET L. |
分类号 |
C07K14/37;(IPC1-7):C12Q1/68;C07H21/04;C12N15/10 |
主分类号 |
C07K14/37 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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