Human tPA production using vectors coding for DHFR protein
摘要
A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.
申请公布号
US5849574(A)
申请公布日期
1998.12.15
申请号
US19950450874
申请日期
1995.05.26
申请人
GENENTECH, INC.
发明人
LEVINSON, ARTHUR D.;PENNICA, DIANE;KOHR, WILLIAM J.;VEHAR, GORDON A.;GOEDDEL, DAVID V.;YELVERTON, ELIZABETH M.;SIMONSEN, CHRISTIAN C.
