A METHOD TO MAP AND ISOLATE REGIONS OF CHROMOSOMES THAT INTERACT OR ASSOCIATE FUNCTIONALLY WITHIN OR BETWEEN CHROMOSOMES IN VIVO

摘要

The present invention relates to a method to identify regions of chromosomes that interact functionally within or between chromosomes which comprises the steps of: a) recombining a linear recipient vector with a target sequence present in at least one chromosome, wherein the recipient vector comprises at least a recipient sequence consisting of a nucleic acid sequence complementary to recombine with the target sequence and a tail consisting of a heterologous nucleic acid sequence; and b) identifying at least one region of the chromosome capable of recombining with the recipient vector by PCR means or by detecting a label and/or a tag on the recipient vector or the target sequence.