METHODS FOR COEXPRESSION OF MORE THAN ONE GENE USING AT LEAST ONE INTERNAL RIBOSOME ENTRY SITE (IRES)

摘要

A primary object of this invention is to provide a method which will enable to express simultaneously desired genes in vitro and in planta. This object is to be accomplished by utilising crTMV RNA sequences upstream of MP gene is termed here as IRESmp. The method of this invention involves the construction of recombinant DNA molecule which comprises of a transcriptional promoter, the first plant-expressible gene linked to the said transcriptional promoter, IRESmp located 3' to the first gene and the second plant-expressible gene located 3' to IRESmp such that the second gene is placed under the translational control of IRESmp. The primary chimeric continuous RNA transcript in positive sense polarity is produced by the transformed cells from plant-expressible promoter. The expression of the first gene takes place by direct translation of the 5'-proximal gene of this mRNA but the translation of the 5'-distal gene of dicistronic mRNA will be promoted by IRESmp.