发明名称 |
RIBOSOME COMPLEXES AS SELECTION PARTICLES FOR IN VITRO DISPLAY AND EVOLUTION OF PROTEINS |
摘要 |
The invention provides a method of diplaying nascent proteins or peptides as complexes with eukaryotic ribosomes and the mRNA encoding the protein or peptide following transcription and translation in vitro, of further selecting complexes carrying a particular nascent protein or peptide by means of binding to a ligand, antigen or antibody, and of subsequently recovering the genetic information encoding the protein or peptide from the selected ribosome complex by reverse transcription and polymerase chain reaction (RT-PCR). The RT-PCR recovery step is carried out directly on the intact ribosome complex, without prior dissociation to release the mRNA, thus contributing to maximal efficiency and sensitivity. The steps of display, selection and recovery can be repeated in consecutive cycles. The method is exemplified using single-chain antibody constructs as antibody-ribosome-mRNA complexes (ARMs).
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申请公布号 |
WO9854312(A1) |
申请公布日期 |
1998.12.03 |
申请号 |
WO1998GB01564 |
申请日期 |
1998.05.28 |
申请人 |
BABRAHAM INSTITUTE;TAUSSIG, MICHAEL, JOHN;HE, MINGYUE |
发明人 |
TAUSSIG, MICHAEL, JOHN;HE, MINGYUE |
分类号 |
C12N15/09;A61K45/00;C07K16/00;C12N15/10;C12P19/34;C12P21/02;C12P21/08;C12Q1/68;(IPC1-7):C12N15/10;C12N15/13 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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