发明名称 |
METHOD FOR MASS PRODUCTION OF ANTIMICROBIAL PEPTIDE |
摘要 |
The present invention provides a method for mass production of an antimicrobial peptide, which comprises the steps of: constructing a fusion gene containing a first gene encoding a negatively charged acidic peptide having at least two cysteine residues and a second gene encoding a positively charged basic antimicrobial peptide; transforming a host microorganism with an expression vector comprising the fusion gene; cultivating the transformed microorganism to express a fusion peptide containing the acidic peptide and the antimicrobial peptide; and, recovering the expressed antimicrobial peptide. In accordance with the present invention, the inhibitory effect of the expressed antimicrobial peptide on the growth of host microorganism can be dramatically minimized by fusing it with the acidic peptide. Accordingly, antimicrobial peptides can be produced massively from a recombinant microorganism regardless of the kind of the antimicrobial peptides.
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申请公布号 |
CA2261569(A1) |
申请公布日期 |
1998.12.03 |
申请号 |
CA19982261569 |
申请日期 |
1998.05.28 |
申请人 |
SAMYANG GENEX CORPORATION;KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
KANG, MIN HYUNG;LEE, JAE HYUN;KIM, SUN-CHANG;LEE, HYUN-SOO;KIM, JEONG HYUN;HONG, SEUNG-SUH |
分类号 |
C12N15/09;C07K7/08;C07K14/435;C07K14/46;C07K14/47;C07K14/815;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/62;C12P21/00;C12P21/02;C12R1/19;(IPC1-7):C12P21/02;C07K19/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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