摘要 |
<p>Devices and methods are provided for the isolation of one or more components of a sample. The self contained device of the subject invention includes a reaction chamber (60), at least one reagent chamber (71) containing a sealed, predetermined amont of reagent, at least one waste chamber (72), an entry port (70), and a means for moving the reaction chamber (60) sequentially into fluid communication with each of these other components. Also provided are methods of using the subject devices to perform procedures in which an initial sample is sequentially subjected to one or more reagent addition and washing steps. The subject devices and methodology are particularly suited to the isolation of nucleic acids from physiological samples.</p> |