| 摘要 |
One or more protein antigens are separated from a first aqueous solution of proteins by injecting the solution into an animal in which the protein antigen(s) elicit antibody response and obtaining therefrom an antiserum, adding to the antiserum a second solution of proteins identical with the first except that the protein antigen(s) to be separated from the first solution is/are absent to precipitate or render permanently soluble all of the protein-antigen complexes common to both solutions and removing the precipitated material to leave the specific antibody or antibodies for the protein antigen(s) to be separated as the only precipitable antibody material remaining in the antiserum, adding the first solution of proteins thereto to precipitate the protein antigen-antibody complexes containing the protein(s) which is/are present only in the first solution, coagulating the precipitated complex(es), exposing the coagulated complex(es) to the first protein solution whereby more protein antigen specific to the antibody present is absorbed on to the coagulated complex, separating the complex and acidifying to free the absorbed protein antigen and separating a solution thereof from the coagulated antibody-protein antigen complex(es). The separated protein may then be used to produce an antiserum in the usual way. A specific example describes the separation of ovalbumin from a mixture of ovalbumin and normal dog serum.ALSO:One or more protein antigens are separated from a first aqueous solution of proteins by injecting the solution into an animal in which the protein antigen(s) elicit antibody response and obtaining therefrom an antiserum, adding to the antiserum a second solution of proteins identical with the first except that the protein antigen(s) to be separated from the first solution is/are absent to precipitate or render permanently soluble all of the protein-antigen complexes common to both solutions and removing the precipitated material to leave the specific antibody or antibodies for the protein antigen(s) to be separated as the only precipitable antibody material remaining in the anti-serum, adding the first solution of proteins thereto to precipitate the protein antigen-antibody complexes containing the protein(s) which is/are present only in the first solution, coagulating the precipitated complex(es), exposing the coagulated complex(es) to the first protein solution whereby more protein antigen specific to the antibody present is absorbed on to the coagulated complex, separating the complex and acidifying to free the absorbed protein antigen and separating a solution thereof from the coagulated antibody-protein antigen complex(es). The separated protein may then be used to produce an antiserum in the usual way. A specific example describes the separation of ovalbumin from a mixture of ovalbumin and normal dog serum.
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