Sorbitol kinase, process for producing the same, and substantially pure microorganism

摘要

PCT No. PCT/JP95/01997 Sec. 371 Date Apr. 18, 1997 Sec. 102(e) Date Apr. 18, 1997 PCT Filed Sep. 29, 1995 PCT Pub. No. WO96/11261 PCT Pub. Date Apr. 18, 1996A sorbitol kinase having at least the following physicochemical properties: (1) Enzymatic action: utilizing mainly ATP, but not substantially phosphoenolpyruvate, as the phosphate donor catalyzing a reaction of generating sorbitol-6-phosphate and ADP from sorbitol and ATP of the formula, sorbitol+ATP->sorbitol-6-phosphate+ADP(2) Substrate specificity: for sorbitol (3) N-terminal amino acid sequence: containing MetArgIleGlyIleAspLeuGlyGlyThr +TR LysThrGluValIleAlaLeuSerGluGln (4) Heat stability stable at least up to 60 DEG C. when a 100 mM Tris-HCl buffer (pH 8.5, containing 0.5 U/ml of sorbitol kinase) is heated for 15 minutes at various temperatures and the residual acitvity is measured.