A water-base antiseptic composition having a consistent and sufficient antiseptic effect and comprising an aqueous solution contaiing at least one component selected from the group consisting of hyaluronic acid, cromoglycic acid, flavin adenine dinucleotide and salts thereof and chlorhexidine gluconate, wherein the gluconate has such a concentration in the aqueous solution that it does not form any insoluble composite with the other components mentioned above and thus maintains the antiseptic effect.