| 摘要 |
<p>The potential usefulness of fibronectin-binding protein (SfbI) of Streptococcus pyogenes as immunological adjuvant was assessed using ovalbumin (OVA) as a model antigen. Mice were immunized by intranasal route either with soluble OVA or OVA covalently coupled to SfbI. Immunization with OVA-SfbI resulted in the elicitation of anti-OVA serum IgG and OVA-specific IgA in lung washes higher than using OVA alone. Spleen cells from OVA-SfbI immunized mice also gave a much stronger proliferative response to in vitro restimulation with soluble OVA. These results demonstrate that SfbI represents a promising mucosal adjuvant able to substantially improve cellular (by both CD4+ and CD8+ T cells), humoral and mucosal responses, when coupled to an antigen administered by intranasal route.</p> |
